Cart summary

You have no items in your shopping cart.

S100-A8/A9 Rabbit Polyclonal Antibody

Catalog Number: orb1974705

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1974705
CategoryAntibodies
DescriptionS100-A8/A9 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityRat
ReactivityHuman, Rat
IsotypeIgG
ImmunogenRecombinant human S100-A8/A9 (1-93&2-114aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW10 kDa
TargetS100A8
UniProt IDP05109
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative names60B8Ag; AI323541; B8Ag; BEE11; CAGA; Calgranulin-A
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
S100-A8/A9 Rabbit Polyclonal Antibody

Sample: Lane 1: Recombinant human S100-A8/A9 protein, His, Primary: Anti-S100-A8/A9 (orb1974705) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 10 kDa, Observed band size: 33 kDa.

S100-A8/A9 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

S100-A8/A9 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

S100-A8/A9 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

S100-A8/A9 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

S100-A8/A9 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

S100-A8/A9 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.