You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1974705 |
---|---|
Category | Antibodies |
Description | S100-A8/A9 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | Recombinant human S100-A8/A9 (1-93&2-114aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 10 kDa |
Target | S100A8 |
UniProt ID | P05109 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | 60B8Ag; AI323541; B8Ag; BEE11; CAGA; Calgranulin-A Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Sample: Lane 1: Recombinant human S100-A8/A9 protein, His, Primary: Anti-S100-A8/A9 (orb1974705) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 10 kDa, Observed band size: 33 kDa.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100-A8 A9) Polyclonal Antibody, Unconjugated (orb1974705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
IHC-Fr, IHC-P, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
AP |
IHC-Fr, IHC-P, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
HRP |
IF, IHC-Fr, IHC-P, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Biotin |
IF | |
Human, Rat | |
Rabbit | |
Polyclonal | |
PerCP/Cy5.5 |