S100A4 Antibody / FSP1

SKU: orb534354

Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A4 may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [RefSeq]

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting points. Titration of the S100A4 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: PS1A4-1R
• Immunogen: A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody (clone PS1A4-1R). Predicted molecular weight ~12 kDa.

IHC testing of FFPE human placenta with recombinant S100A4 antibody (clone PS1A4-1R). Required HIER: steam sections in 10 mM citrate buffer, pH6, for 10-20 min and allow to cool before testing.

FACS testing of permeabilized human A549 cells with recombinant S100A4 antibody (blue, clone PS1A4-1R) and isotype control (red).

Quick Database Links

UniProt

P26447