S100A12 Antibody / CGRP

• Catalog Number: orb1824377
• Category: Antibodies
• Description: The family of EF-hand type Ca2+-binding proteins includes Calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 Alpha and beta, Calgranulins A (also designated MRP8), B (also designated MRP14) and C (S100A12, CGRP) and the parvalbumin family members, including parvalbumin Alpha and parvalbumin beta (also designated oncomodulin). Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 Alpha and beta are present in a variety of other tissues, and Calbindin is present in intestine and kidney. Parvalbumin Alpha is also found in fast-contracting/relaxing skeletal muscle fibers and parvalbumin beta is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in leydig cells and the testis. These proteins are thought to play a role in hormone production and spermatogenesis. Calgranulin is expressed in macrophages and epithelial cells.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 1-95) from the human protein was used as the immunogen for the CGRP antibody.
• UniProt ID: P80511
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: S100A12/8952R
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human spleen tissue with CGRP antibody (clone S100A12/8952R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free S100A12 antibody (clone S100A12/8952R) as confirmation of integrity and purity.