S100A11 Antibody

SKU: orb1274450

Description

S100A11 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human S100A11 (NP_005611.1).
• Target: S100A11
• Molecular Weight: Observed: 12kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

HEL-S-43, MLN7S100C, protein S100-A11, MLN 7calgizzarin, epididymis secretory protein Li 43, metastatic lymph node gene 70 protein, protein S100-C

Western blot analysis of extracts of various cell lines, using S100A11 antibody (orb1274450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts from normal (control) and S100A11 knockout (KO) HeLa cells, using S100A11 antibody (orb1274450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human kidney using S100A11 antibody (orb1274450) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using S100A11 antibody (orb1274450). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

S100A11

UniProt

P31949