S100A11 Rabbit Polyclonal Antibody

• Catalog Number: orb1974598
• Category: Antibodies
• Description: S100A11 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, IP, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human S100A11 protein (1-105/105aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1000, IP=1:50-200, ICC/IF=0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 13 kDa
• Target: S100A11
• UniProt ID: P31949
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Calgizzarin; Metastatic lymph node gene 70 protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100A11) Polyclonal Antibody, Unconjugated (orb1974598) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (S100A11) Polyclonal Antibody, Unconjugated (orb1974598) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human U-2 OS cell lysates, Lane 3: Human DU145 cell lysates, Lane 4: Human MCF-7 cell lysates, Primary: Anti-S100A11 (orb1974598) at 1/400 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13 kDa, Observed band size: 10 kDa.