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Catalog Number | orb1239947 |
---|---|
Category | Antibodies |
Description | RSPO1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Anti-RSPO1 antibody (orb1239947) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1. |
Concentration | 1 mg/mL |
Dilution range | WB: 0.5-2 μg/mL; IHC: 2.5-5 μg/mL; IF: 5 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 29kDObserved: 36 kD (Post-modification: 1 N-linked glycosylation) |
Target | RSPO1 |
UniProt ID | Q2MKA7 |
NCBI | NP_001033722 |
Storage | RSPO1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | RSPO1 Antibody: RSPO, CRISTIN3, R-spondin-1, Roof Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: RSPO1, orb1239947 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 30 ng of human RSPO1 recombinant protein per lane. Antibodies: RSPO1, orb1239947 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL, Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Spleen. Loading: 15 µg of lysates per lane. Antibodies: RSPO1, orb1239947 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Heart. Loading: 15 µg of lysates per lane. Antibodies: RSPO1, orb1239947 (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of RSPO1 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling RSPO1 with orb1239947 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).
Immunohistochemistry Validation of RSPO1 in Human Heart. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-RSPO1 antibody (orb1239947) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (orb1239947) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (orb1239947) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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