RSPO1 Antibody

• Catalog Number: orb1239947
• Category: Antibodies
• Description: RSPO1 Antibody
• Target: RSPO1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: RSPO1 Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-RSPO1 antibody (orb1239947) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1.
• UniProt ID: Q2MKA7
• MW: Predicted: 29kDObserved: 36 kD (Post-modification: 1 N-linked glycosylation)
• Tested applications: ELISA, IF, IHC-P, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RSPO1 Antibody: RSPO, CRISTIN3, R-spondin-1, Roof
• Note: For research use only
• NCBI: NP_001033722

Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: RSPO1, orb1239947 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human RSPO1 recombinant protein per lane. Antibodies: RSPO1, orb1239947 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL, Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Spleen. Loading: 15 µg of lysates per lane. Antibodies: RSPO1, orb1239947 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Heart. Loading: 15 µg of lysates per lane. Antibodies: RSPO1, orb1239947 (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of RSPO1 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling RSPO1 with orb1239947 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).

Immunohistochemistry Validation of RSPO1 in Human Heart. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-RSPO1 antibody (orb1239947) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (orb1239947) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (orb1239947) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.