RSPO1 Rabbit Polyclonal Antibody

SKU: orb526678

Description

Research Area

Stem Cells

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Rat
Predicted Reactivity	Canine, Human, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human RSPO1 (21-120/263aa)
Target	RSPO1
Molecular Weight	27 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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R-spondin-1; R spondin1; CR spondin 1; RISTIN3; FLJ40906; hRspo1; R spondin homolog(Xenopus laevis); R spondin homolog; Roof plate specific spondin; Roof plate-specific spondin-1; RP11-566C13.1; RSPO; RSPO1_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RSPO1) Polyclonal Antibody, Unconjugated (orb526678) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

RSPO1

UniProt

Q2MKA7

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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IF (1)

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Human (1)

Alessandro Cherubini # 1, Clelia Pistoni # 2 3, Maria Chiara Iachini # 2, Cecilia Mei 2 4, Francesco Rusconi 2, Valeria Peli 2, Mario Barilani 2, Dorian Tace 2, Noemi Elia 2, Fabio Lepore 5, Vittoria Caporale 6, Lorenzo Piemonti 7, Lorenza Lazzari 8 R-spondins secreted by human pancreas-derived mesenchymal stromal cells support pancreatic organoid proliferation Cell Mol Life Sci, (2025)

PubMed: 40111532

Applications

Reactivity

Human