RSPO1 Rabbit Polyclonal Antibody

SKU: orb526678

Description

RSPO1 Rabbit Polyclonal Antibody

Research Area

Stem Cells

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Canine, Human, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RSPO1 (21-120/263aa)
• Target: RSPO1
• Molecular Weight: 27 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

R-spondin-1; R spondin1; CR spondin 1; RISTIN3; FLJ40906; hRspo1; R spondin homolog(Xenopus laevis); R spondin homolog; Roof plate specific spondin; Roof plate-specific spondin-1; RP11-566C13.1; RSPO; RSPO1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RSPO1) Polyclonal Antibody, Unconjugated (orb526678) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

RSPO1

UniProt

Q2MKA7

Alessandro Cherubini # 1, Clelia Pistoni # 2 3, Maria Chiara Iachini # 2, Cecilia Mei 2 4, Francesco Rusconi 2, Valeria Peli 2, Mario Barilani 2, Dorian Tace 2, Noemi Elia 2, Fabio Lepore 5, Vittoria Caporale 6, Lorenzo Piemonti 7, Lorenza Lazzari 8 R-spondins secreted by human pancreas-derived mesenchymal stromal cells support pancreatic organoid proliferation Cell Mol Life Sci, (2025)

PubMed: 40111532

Applications

IF

Reactivity

Human