RSPO1 Rabbit Polyclonal Antibody

• Catalog Number: orb526678
• Category: Antibodies
• Description: RSPO1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Canine, Human, Porcine, Rabbit
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RSPO1 (21-120/263aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: RSPO1
• UniProt ID: Q2MKA7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: R-spondin-1; R spondin1; CR spondin 1; RISTIN3; FL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Alessandro Cherubini # 1, Clelia Pistoni # 2 3, Maria Chiara Iachini # 2, Cecilia Mei 2 4, Francesco Rusconi 2, Valeria Peli 2, Mario Barilani 2, Dorian Tace 2, Noemi Elia 2, Fabio Lepore 5, Vittoria Caporale 6, Lorenzo Piemonti 7, Lorenza Lazzari 8 R-spondins secreted by human pancreas-derived mesenchymal stromal cells support pancreatic organoid proliferation Cell Mol Life Sci, (2025)

PubMed: 40111532

Applications

IF

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RSPO1) Polyclonal Antibody, Unconjugated (orb526678) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.