RS-1

SKU: orb1685531

Description

RS-1 is a small molecule activator of the RAD51 recombinase, enhancing its DNA strand invasion activity. This property significantly improves the efficiency of CRISPR/Cas9-mediated homology-directed repair (HDR) for precise gene knock-in applications in both cellular and animal models.

Research Area

Molecular Biology, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 312756-74-4
• MW: 524.23
• Purity: >98%
• Formula: C₂₀H₁₆Br₂N₂O₃S
• SMILES: Brc1ccc(NC(=O)c2ccc(Br)c(c2)S(=O)(=O)NCc2ccccc2)cc1
• Target: DNA
• Solubility: Soluble in DMSO (up to 30 mg/ml)

Bioactivity

• In Vitro:
  RS-1 serves as an activator of RAD51, enhancing the binding affinity of hRAD51 to DNA, with a dissociation constant (Kd) between 48 nM and 107 nM, in conditions containing ATP or ADP and even in the absence of nucleotide cofactors, without inhibiting ATPase activity of RAD51. At a concentration of 20 μM, RS-1 alters the length and helical pitch of hRAD51-DNA complexes. Furthermore, RS-1 progressively augments the strand assimilation activity of hRAD51 across a range of concentrations (0, 1, 5, 10, 15, 20, and 25 μM). At 7.5 μM, it bolsters human cells' resilience to cross-linking agents used in chemotherapy. In addition, RS-1 enhances Cas9-mediated gene editing efficiency in rabbit embryos at concentrations of 0, 7.5, and 15 μM.
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Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRISPR/Cas9, DNA Synthesis, DNASynthesis, inhibit, Inhibitor, RAD51, RNASynthesis, RNA Synthesis, RS 1, RS1, RS-1

Compound Identifiers

PubChem CID

1039737