RS-1

SKU: orb1225342

Description

RS-1 is a homology-directed repair (HDR) enhancer; enhances HDR 3- to 6-fold depending on the locus and transfection method; shows effectivity on improving knock-in rates in both the TALEN- and the CRISPR/Cas9-mediated genome-editing systems.

Key Properties

• CAS Number: 312756-74-4
• MW: 524.2256
• Purity: >98% (HPLC)
• Formula: C₂₀H₁₆Br₂N₂O₃S
• SMILES: O=C(NC1=CC=C(Br)C=C1)C2=CC=C(Br)C(S(=O)(NCC3=CC=CC=C3)=O)=C2
• Target: CRISPR/Cas9
• Solubility: 10 mM in DMSO

Bioactivity

• In Vitro:
  RS-1 is a RAD51 activator, stimulating binding of hRAD51 to DNA with Kd ranging from 48 nM to 107 nM in the presence of ATP or ADP and in the absence of a nucleotide cofactor, and such an effect is not via inhibiting its ATPase activity. RS-1 (20 μM) affects the length and helical pitch of hRAD51 protein-DNA complexes. RS-1 (0, 1, 5, 10, 15, 20, and 25 μM) stimulates strand assimilation activity of hRAD51. RS-1 (7.5 μM) promotes resistance of human cells to cross-linking chemotherapy. RS-1 (0, 7.5, 15 μM) increases Cas9-mediated knock-in efficiencies in rabbit embryos.
  Read more...

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RS1 | RS 1

Quick Database Links

Gene Symbol

CRISPR/Cas9