RRAD Antibody

SKU: orb1248716

Description

RRAD Antibody

Images & Validation

• Tested Applications: ELISA, KO/KD Validated, WB
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Heart lysates and no 37kDa in the KO lysates (calculated MW of 33.2kDa according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than Western blot. In transfected HEK293 transiently expressing Mouse Rrad (HA tagged), a band of approx. 45kDa is observed. No bands are observed in transfected HEK293 transiently expressing related Mouse GTPases. Recommended concentration: 0.5-2µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HRRSMPVDERDLQ
• Target: RRAD
• Molecular Weight: Approx 37 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RRAD, Ras-related associated with diabetes, RAD, RAD1, REM3, GTP-binding protein RAD, OTTHUMP00000174805, RAS (RAD and GEM) like GTP binding 3, ras associated with diabetes

A): orb1248716 (0.5 ug/ml) staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer) and compared with an HA-specific antibody. B): orb1248716 (0.5 ug/ml) staining of WT and KO lysates of Mouse Heart.

Quick Database Links

Gene Symbol

RRAD

UniProt

P55042

RefSeq (Protein):NP_004156.1