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RPSA Antibody / 40S Ribosomal protein SA

Catalog Number: orb606807

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606807
CategoryAntibodies
DescriptionLaminins, a family of extracellular matrix glycoproteins, are the major non-collagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. Reportedly, level of laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenRecombinant full length protein was used as the immunogen for the RPSA antibody.
UniProt IDP08865
Tested applicationsFACS, IF, IHC-P, WB
Dilution rangeFlow cytometry: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesOptimal dilution of the RPSA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberRPSA/2699
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis RPSA antibody is available for research use only.
NoteFor research use only
RPSA Antibody / 40S Ribosomal protein SA

IHC testing of FFPE human cervical carcinoma stained with RPSA antibody. Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

RPSA Antibody / 40S Ribosomal protein SA

IHC testing of FFPE human colon carcinoma stained with RPSA antibody. Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

RPSA Antibody / 40S Ribosomal protein SA

IHC testing of FFPE human breast carcinoma stained with RPSA antibody. Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

RPSA Antibody / 40S Ribosomal protein SA

IHC testing of FFPE human basal cell carcinoma stained with RPSA antibody. Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

RPSA Antibody / 40S Ribosomal protein SA

IHC testing of FFPE human tonsil stained with RPSA antibody. Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

RPSA Antibody / 40S Ribosomal protein SA

Western blot testing of human MCF-7, MDA-MB-453, SK-BR3, and HeLa cell lysate with RPSA antibody. Routinely observed molecular weight: 37-40 kDa and 67 kDa.

RPSA Antibody / 40S Ribosomal protein SA

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using RPSA antibody. These results demonstrate the foremost specificity of the RPSA/2699 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

RPSA Antibody / 40S Ribosomal protein SA

Immunofluorescent staining of human HeLa cells with RPSA antibody (green, clone RPSA/2699) and Reddot nuclear stain (red).

RPSA Antibody / 40S Ribosomal protein SA

Immunofluorescent staining of paraformaldehyde-Raji cells with RPSA antibody (green, clone RPSA/2699) and Reddot nuclear stain (red).

RPSA Antibody / 40S Ribosomal protein SA

FACS staining of paraformaldehyde-Raji cells with RPSA antibody (clone RPSA/2699); Red = isotype control, Blue = RPSA antibody.

  • RPSA Antibody / 40S Ribosomal protein SA [orb534304]

    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • RPSA Antibody / 40S Ribosomal protein SA [orb1151760]

    IHC-P

    Human

    Mouse

    Recombinant

    Unconjugated

    20 μg, 100 μg