RPSA Antibody / 40S Ribosomal protein SA

SKU: orb534304

Description

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. [UniProt]

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting points. Titration of the RPSA antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: LMNR-1
• Immunogen: Recombinant full length protein was used as the immunogen for the RPSA antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human HeLa cell lysate with RPSA antibody (clone LMNR-1). Routinely observed molecular weight: 37-40 kDa and 67 kDa.

IHC testing of FFPE human tonsil stained with RPSA antibody (clone LMNR-1). Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human cervical carcinoma stained with RPSA antibody (clone LMNR-1). Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human HeLa cells with RPSA antibody (green, clone LMNR-1) and Reddot nuclear stain (red).

FACS staining of PFA-fixed Raji cells with RPSA antibody (clone LMNR-1); Red = isotype control, Blue = RPSA antibody.

Quick Database Links

UniProt

P08865