RPS6 Antibody (N-term)

• Catalog Number: orb1927147
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This RPS6 antibody is generated from a mouse immunized with RPS6 recombinant protein.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse, Rabbit
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: P62753
• MW: 28681 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:25, IF: 1:25, WB: 1:2000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Clone Number: 981CT2.1.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 40S ribosomal protein S6, Phosphoprotein NP33, RPS
• Note: For research use only

Western blot analysis of lysate from 293 cell line, using RPS6 Antibody (N-term). AM8431a was diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysate at 35μg.

Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). AM8431a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). AM8431a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). AM8431a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). AM8431a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate (from left to right), using RPS6 Antibody (N-term). Diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.