RPS6 Antibody (N-term)

SKU: orb1927147

Description

RPS6 Antibody (N-term)

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: FC - 1:25, IF - 1:25, IHC-P - 1:100-500, WB - 1:2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rabbit

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Molecular Weight: 28681 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of lysate from 293 cell line, using RPS6 Antibody (N-term). AM8431a was diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysate at 35μg.

Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). AM8431a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). AM8431a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). AM8431a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). AM8431a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate (from left to right), using RPS6 Antibody (N-term). Diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Quick Database Links

UniProt

P62753