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RPS6 (N-term) Mouse Monoclonal Antibody

Catalog Number: orb559232

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb559232
CategoryAntibodies
DescriptionRPS6 (N-term) Mouse Monoclonal Antibody
Species/HostMouse
ClonalityMonoclonal
Clone Number3D5
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant human RPS6
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:200-1000, IHC-P=1:20-200, IHC-F=1:20-200, IF=1:20-200
Form/AppearanceLiquid
ConjugationUnconjugated
MW29 kDa
TargetRPS6
UniProt IDP62753
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative names40S ribosomal protein S6; Air8; DS6; Hemocytes enl
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NoteFor research use only
Expiration Date12 months from date of receipt.
RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human brain section), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RPS6) Monoclonal Antibody, Unconjugated (orb559232) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (orb559232) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

RPS6 (N-term) Mouse Monoclonal Antibody

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human A431 cell lysates, Lane 4: Human HepG2 cell lysates, Primary: Anti-RPS6 (N-term) (orb559232) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 29 kDa, Observed band size: 32 kDa.

  • RPS6 Antibody (N-term) [orb1788410]

    FC,  IF,  IHC-P,  WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μl
  • RPS6 Antibody (N-term) [orb1927147]

    FC,  IF,  IHC-P,  WB

    Mouse, Rabbit

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μl, 50 μl