RPL27 Antibody

• Catalog Number: orb1260621
• Category: Antibodies
• Description: RPL27 Antibody
• Target: RPL27
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human RPL27 (NP_000979.1).
• UniProt ID: P61353
• MW: Observed: 16kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 60S ribosomal protein L27, RPL27
• Note: For research use only

Western blot analysis of extracts of various cell lines, using RPL27 antibody (orb1260621) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunofluorescence analysis of C6 cells using RPL27 Polyclonal Antibody (orb1260621) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using RPL27 Polyclonal Antibody (orb1260621) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using RPL27 Polyclonal Antibody (orb1260621) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.