RPL27 Antibody (C-term)

• Catalog Number: orb1936916
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32393
• Tested applications: IHC-P, WB
• Predicted Reactivity: Porcine, Rabbit, Rat, Zebrafish
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:4000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 15798 Da
• Target: This RPL27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the C-terminal region of human RPL27.
• UniProt ID: P61353
• NCBI: NP_000979.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 60S ribosomal protein L27, RPL27
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-RPL27 Antibody (C-term) at 1:4000 dilution + RPMI 8226 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 16 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

RPL27 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RPL27 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

RPL27 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the RPL27 antibody detected the RPL27 protein (arrow).