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RPE Antibody

Catalog Number: orb1259790

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb1259790
CategoryAntibodies
DescriptionRPE Antibody
ClonalityPolyclonal
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human RPE (NP_954699.1).
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MWObserved: 25kDa
TargetRPE
UniProt IDQ96AT9
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Alternative namesRPE2-1, ribulose-phosphate 3-epimerase
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NoteFor research use only
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Expiration Date12 months from date of receipt.
RPE Antibody

Western blot analysis of extracts of various cell lines, using RPE antibody (orb1259790) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

RPE Antibody

Immunofluorescence analysis of L929 cells using RPE Polyclonal Antibody (orb1259790) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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