RNA polymerase II subunit B Antibody

SKU: orb556044

Description

Rabbit polyclonal antibody to RPB2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ChIP, ICC, IHC-P, WB
• Dilution Range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Reactivity: Human, Mouse
• Application Notes:
  Positive control: H1299 , BCL-1

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
• Target: RPB2
• Molecular Weight: 134
• Purity: Purified by antigen-affinity chromatography.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Buffer/Preservatives: 0.1M Tris, 0.1M Glycine, 20% Glycerol, 0.01% Thimerosal.
• Concentration: 0.32 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RNA polymerase II subunit B , POL2RB , RPB2 , hRPB140

Immunofluorescence analysis of HeLa cells using RPB2 antibody

Immunohistochemical staining of NCIN87 xenograft using RPB2 antibody

Immunohistochemical staining of human breast carcinoma using RPB2 antibody

Western blot analysis of H1299 cells using RPB2 antibody

Western blot analysis of BCL-1 cells using RPB2 antibody

Western blot analysis of H1299 cells using PPEF1 antibody

Quick Database Links

Gene Symbol

RPB2

UniProt

P30876