Anti-POLR2B Antibody

• Catalog Number: orb412404
• Category: Antibodies
• Description: Rabbit polyclonal antibody to POLR2B
• Target: POLR2B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant full length protein of human POLR2B
• UniProt ID: P30876, Q8CFI7
• Tested applications: IH, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-DNA-directed RNA polymerase II subunit RPB2 a
• Note: For research use only
• Entrez: 5431, 231329

Western blot analysis of POLR2B expression in SHSY5Y (A), HepG2 (B), mouse brain (C), rat ovary (D) whole cell lysates. (Predicted band size: 133 kD; Observed band size: 134 kD)

Immunohistochemical analysis of POLR2B staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.