Anti-POLR2B Antibody

• Catalog Number: orb412404
• Category: Antibodies
• Description: Rabbit polyclonal antibody to POLR2B
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human POLR2B
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: POLR2B
• Entrez: 5431, 231329
• UniProt ID: P30876, Q8CFI7
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-DNA-directed RNA polymerase II subunit RPB2 a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of POLR2B expression in SHSY5Y (A), HepG2 (B), mouse brain (C), rat ovary (D) whole cell lysates. (Predicted band size: 133 kD; Observed band size: 134 kD)

Immunohistochemical analysis of POLR2B staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.