ROS1 Antibody

• Catalog Number: orb1823069
• Category: Antibodies
• Description: ROS1, an orphan receptor tyrosine kinase of the insulin receptor family, was initially identified as a homolog of v-ros from the UR2 sarcoma virus. ROS1 consists of a large extracellular domain that is composed of six fibronectin repeats, a transmembrane domain, and an intracellular kinase domain. While the function of ROS1 is undefined, it has been shown to play an important role in differentiation of epididymal epithelium. The first oncogenic fusion of ROS1, FIG-ROS1, was initially identified by research studies in glioblastoma. Investigators have found additional oncogenic ROS1 fusion proteins in NSCLC (at a frequency of ~1.6%), where the ROS1 kinase domain is fused to the amino-terminal region of a number of different proteins, including CD74 and SLC34A2.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ROS1/8268R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant fragment corresponding to the cytoplasmic domain of human ROS1 protein was used as the immunogen for the ROS1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This ROS1 antibody is available for research use only.
• UniProt ID: P08922
• Storage: Aliquot the ROS1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human epididymis tissue with ROS1 antibody (clone ROS1/8268R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free ROS1 antibody (clone ROS1/8268R) as confirmation of integrity and purity.