ROS1 Rabbit Polyclonal Antibody

• Catalog Number: orb13678
• Category: Antibodies
• Description: ROS1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ROS1 (2231-2347/2347aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 258 kDa
• Target: ROS1
• UniProt ID: P08922
• RRID: AB_10750989
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ROS; ros protein; c ros; c-ros; c ros1; MCF3; Onco
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Benetti, F. et al. The presence of osteocalcin, osteopontin and reactive oxygen species-positive cells in pulp tissue after dental bleaching Int Endod J, 52, 665-675 (2019)

PubMed: 30488465

Applications

IHC

Reactivity

Rat

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-ROS1 antibody (orb13678), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebellum (Rat) Lysate at 40 ug, Lane 3: Pituitary (Rat) Lysate at 40 ug, Lane 4: Siha (Human) Cell Lysate at 30 ug, Lane 5: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-ROS1 (orb13678) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 258 kD, Observed band size: > 250/110 kD.