ROS1 Rabbit Polyclonal Antibody

SKU: orb13678

Description

ROS1 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Growth Factors, Proto-oncogenes, Receptor Tyrosne Kinases

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ROS1 (2231-2347/2347aa)
• Target: ROS1
• Molecular Weight: 258 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ROS; ros protein; c ros; c-ros; c ros1; MCF3; Oncogene ROS; OTTHUMP00000040389; Proto oncogene c ros 1 protein; Proto oncogene tyrosine protein kinase ROS; Proto oncogene tyrosine protein kinase ROS precursor; Ros1; ROS1C; Transmembrane tyrosine specific protein kinase; v ros avian UR2 sarcoma virus oncogene homolog 1; v ros UR2 sarcoma virus oncogene homolog 1; ROS1_HUMAN; Proto-oncogene tyrosine-protein kinase ROS; Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Receptor tyrosine kinase c-ros oncogene 1; c-Ros receptor tyrosine kinase.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-ROS1 antibody (orb13678), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebellum (Rat) Lysate at 40 ug, Lane 3: Pituitary (Rat) Lysate at 40 ug, Lane 4: Siha (Human) Cell Lysate at 30 ug, Lane 5: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-ROS1 (orb13678) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 258 kD, Observed band size: > 250/110 kD.

Quick Database Links

Gene Symbol

ROS1

UniProt

P08922

References

Benetti, F. et al. The presence of osteocalcin, osteopontin and reactive oxygen species-positive cells in pulp tissue after dental bleaching Int Endod J, 52, 665-675 (2019)

PubMed: 30488465

Applications

IHC

Reactivity

Rat