ROR1 Antibody

• Catalog Number: orb1928997
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q01973
• MW: 104283 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:4000, WB: 1:4000, IHC-P: 1:100, IHC-P: 1:100, FC: 1:25
• Clone Number: RB14753
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tyrosine-protein kinase transmembrane receptor ROR
• Note: For research use only
• NCBI: NP_001077061.1, NP_005003.2

Anti-ROR1 Antibody at 1:4000 dilution + PANC-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded Human heart tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-ROR1 Antibody at 1:4000 dilution. Lane 1: K562 whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Lane 3: NCI-H226 whole cell lysate. Lane 4: A549 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: Mouse kidney tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing A549 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.