ROCK1 Antibody, KO Validated

• Catalog Number: orb1274559
• Category: Antibodies
• Description: ROCK1 Antibody, KO Validated
• Target: ROCK1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
• UniProt ID: Q13464
• MW: Observed: 158kDa
• Tested applications: IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IP: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated p
• Note: For research use only

Western blot analysis of extracts of various cell lines, using ROCK1 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat heart using ROCK1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ROCK1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using ROCK1 antibody at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody. Western blot was performed from the immunoprecipitate using ROCK1 antibody at a dilition of 1:1000.