ROCK1 Antibody

SKU: orb1274559

Description

ROCK1 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
• Target: ROCK1
• Molecular Weight: Observed: 158kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, ROCK-I

Western blot analysis of extracts of various cell lines, using ROCK1 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat heart using ROCK1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ROCK1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using ROCK1 antibody at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody. Western blot was performed from the immunoprecipitate using ROCK1 antibody at a dilition of 1:1000.

Quick Database Links

Gene Symbol

ROCK1

UniProt

Q13464