ROCK1 antibody [N1N2], N-term

SKU: orb556838

Description

Rabbit polyclonal antibody to ROCK1

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ICC, IHC-P, IP, WB
• Dilution Range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Positive control: rat brain , NIH-3T3 , C2Cl2 , HeLa

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.
• Target: ROCK1
• Molecular Weight: 158
• Purity: Purified by antigen-affinity chromatography.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Form/Appearance: Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Buffer/Preservatives: PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300.
• Concentration: 0.78 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I

Immunofluorescence analysis of HeLa cells using ROCK1 antibody

Immunohistochemical staining of human breast carcinoma using ROCK1 antibody

Immunoprecipitation analysis of 293T whole cell using ROCK1 antibody

Western blot analysis of Various whole cell using ROCK1 antibody

Western blot analysis of NIH-3T3 whole cell(Lane 1), C2Cl2 whole cell(Lane 2) using ROCK1 antibody

Western blot analysis of rat brain lysate using ROCK1 antibody

Western blot analysis of Various whole cell using Thymidylate synthase antibody

Quick Database Links

Gene Symbol

ROCK1

UniProt

Q13464