ROBO1 Antibody

SKU: orb1539102

Description

ROBO1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, IP, WB
• Dilution Range: ELISA (1:30000 - 1:60000), IF, IHC, IHC-P (2.5 µg/ml), IP, WB (1:500 - 1:3000)
• Reactivity: Canine, Human, Mouse, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.
• Target: ROBO1
• Purification: Affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ROBO1, Deleted in U twenty twenty, H-Robo-1, Roundabout 1, SAX3, DUTT1, Roundabout homolog 1

Quick Database Links

Gene Symbol

ROBO1