ROBO1 Antibody

• Catalog Number: orb1537963
• Category: Antibodies
• Description: ROBO1 Antibody
• Target: ROBO1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Gallus, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rat, Xenopus
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Immunogen: Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1NP_598334.1.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: IF (10 µg/ml), IHC, IHC-P (2 µg/ml), Peptide-ELISA (1:8000), WB (1 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B3011 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3011 was determined to be 2 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ROBO1, Deleted in U twenty twenty, H-Robo-1, Round
• Note: For research use only

Goat Anti-ROBO1 / DUTT1 (Internal) Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat Anti-ROBO1 / DUTT1 (Internal) Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.