ROBO1 Antibody

SKU: orb1537963

Description

ROBO1 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: IF (10 µg/ml), IHC, IHC-P (2 µg/ml), Peptide-ELISA (1:8000), WB (1 µg/ml)
• Reactivity: Bovine, Canine, Equine, Gallus, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rat, Xenopus
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1NP_598334.1.
• Target: ROBO1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ROBO1, Deleted in U twenty twenty, H-Robo-1, Roundabout 1, SAX3, DUTT1, Roundabout homolog 1

Goat Anti-ROBO1 / DUTT1 (Internal) Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat Anti-ROBO1 / DUTT1 (Internal) Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.

Quick Database Links

Gene Symbol

ROBO1