ROBO1 Antibody

• Catalog Number: orb1259569
• Category: Antibodies
• Description: ROBO1 Antibody
• Target: ROBO1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1452-1651 of human ROBO1 (NP_002932.1).
• UniProt ID: Q9Y6N7
• MW: Observed: 270kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ROBO1, roundabout, axon guidance receptor, homolog
• Note: For research use only

Western blot analysis of extracts of various cell lines, using ROBO1 antibody (orb1259569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using ROBO1 antibody (orb1259569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of U-2 OS cells using ROBO1 Polyclonal Antibody (orb1259569) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.