Robo1 Antibody

• Catalog Number: orb1247001
• Category: Antibodies
• Description: Robo1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: C-QKARPAKKQKHQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 200kDa band observed in fetal Mouse Brain lysates (calculated MW of 176kDa according to NP_062286.2). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Mouse Testis. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Robo1
• UniProt ID: O89026
• NCBI: NP_062286.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Robo1, roundabout homolog 1 (Drosophila) , AW49463
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 200kDa band observed in fetal Mouse Brain lysates (calculated MW of 176kDa according to NP_062286.2). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Mouse Testis. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247001 (0.05 ug/ml) staining of fetal Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247001 (5 ug/ml) staining of paraffin embedded Mouse Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.