RNF7 Antibody

• Catalog Number: orb1249385
• Category: Antibodies
• Description: RNF7 Antibody
• Target: RNF7
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: DACLRCQAENKQE
• UniProt ID: Q9UBF6
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 11kDa band observed in Human Human Heart lysates (calculated MW of 12.7kDa according to NP_055060.1). Recommended concentration: 1-3ug/ml. An additional band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RNF7, ring finger protein 7, CKBBP1, ROC2, SAG, OT
• Research Area: Cell Biology
• Note: For research use only
• NCBI: NP_055060.1

orb1249385 (1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249385 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.