RNF7 Antibody (N-term)

• Catalog Number: orb1926253
• Category: Antibodies
• Description: RNF7 Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UBF6
• MW: 12683 Da
• Tested applications: IHC-P, WB
• Dilution range: IHC-P - 1:100-500, WB - 1:8000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RBX2, ROC2, SAG
• Research Area: Cell Biology, Metabolism Research
• Note: For research use only

All lanes: Anti-RNF7 Antibody (N-term) at 1:8000 dilution. Lane 1: human heart lysates. Lane 2: human liver lysates. Lane 3: human skeletal muscle lysates. Lane 4: human testis lysates. Lane 5: mouse heart lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 13 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining RNF7 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.