Rkhd4 Antibody

• Catalog Number: orb1239124
• Category: Antibodies
• Description: Rkhd4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Rkhd4 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Rkhd4.The immunogen is located within amino acids 420 - 470 of Rkhd4.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 54 kD Observed: 54 kD
• Target: MEX3A
• UniProt ID: A1L020
• NCBI: A1L020
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Rkhd4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Rkhd4 Antibody: RKHD4, MEX-3A, RKHD4, RNA-binding
• Note: For research use only
• Application notes: Rkhd4 antibody can be used for detection of Rkhd4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of RKHD4 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-RKHD4 antibody (orb1239124) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of RKHD4 in Mouse lung. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RKHD4 antibody (orb1239124) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of RKHD4 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RKHD4 antibody (orb1239124) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

WB Validation in Human, Mouse and Rat Samples. Loading: 15 µg of lysate Antibodies: RKHD4, orb1239124, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.