Rituximab Antibody

SKU: orb1297804

Description

Rituximab

Research Area

Immunology & Inflammation

Key Properties

• CAS Number: 174722-31-7
• MW: 148 kDa
• Purity: 98.20%
• Target: Immunology/Inflammation related,Others

Bioactivity

• Target IC50:
  RL cells:1.5 ± 0.1 nM (0.22 mg/ml)|Ramos cells:1 ± 0.1 nM (0.15 mg/ml)
• In Vivo:
  METHODS: 100 mg of Rituximab was administered over 4 h. Rituximab was then infused in a regimen of 3 weekly infusions for 4 weeks, and caspase-3 and PARP expression and protein hydrolysis processes were examined in patients. RESULTS Rituximab induced cleavage of caspase-3, followed by the appearance of a 17-kd cleavage product corresponding to the large subunit of the catalytically active protease, and cleavage of PARP, a known substrate of cysteine asparaginase, was also detected.
• In Vitro:
  METHODS: B cells were treated with 0, 2.5, 5 and 10 μg/mL Rituximab to observe the effect of Rituximab on B cell proliferation. RESULTS There was an optimal inhibition of B cell proliferation at a concentration of 5 μg/mL.
• Cell Research:
  Objective: Complement-mediated cell lysis. Concentrations: 0, 0.02, 0.2, 2, 20 μg/mL. Incubation Time: 2 h. Method: Lymphoma B cells were purified from lymph node biopsies of patients with B-cell lymphoma. Rituximab was added to tumor cells (1×106 /mL) in complete medium supplemented by human serum, inactivated or not by incubation at 56°C for 30 minutes. After 2 hours at 37°C, cell lysis was determined by PI staining of cells and analysis by flow cytometry.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FcR, Rituximab

Quick Database Links

Gene Symbol

Immunology/Inflammation related,Others