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CD79a Antibody Cocktail

Catalog Number: orb248285

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb248285
CategoryAntibodies
DescriptionA disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. CD79a antibody is generally used to complement CD20 antibody especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody cocktail will stain many of the same lymphomas as CD20 antibody, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is CD20 antibody. CD79a antibody also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenA synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein (JCB117 and HM47/A9) was used as the immunogen for this antibody cocktail.
Tested applicationsFACS, IF, IHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberJCB117 + HM47/A9
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the CD79a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Hazard InformationThis CD79a antibody is available for research use only.
NoteFor research use only
Entrez973
CD79a Antibody Cocktail

IHC testing of FFPE human tonsil (10X) stained with CD79a antibody cocktail (clone JCB117 + HM47/A9).

CD79a Antibody Cocktail

IHC testing of FFPE human tonsil (10X) stained with CD79a antibody cocktail (clone JCB117 + HM47/A9).

CD79a Antibody Cocktail

Flow cytometry testing of human Raji cells with CD79a antibody cocktail (clone JCB117 + HM47/A9); Red = isotype control, Blue = CD79a antibody cocktail.

CD79a Antibody Cocktail

Western blot testing of human Raji cell lysate with CD79a antibody cocktail (clone JCB117 + HM47/A9). Expected molecular weight: 25-47 kDa depending on glycosylation level.

CD79a Antibody Cocktail

Immunofluorescent staining of PFA-fixed human Raji cells with CD79a antibody cocktail (clone JCB117 + HM47/A9, green) and Reddot nuclear stain (red).

CD79a Antibody Cocktail

SDS-PAGE analysis of purified, BSA-free CD79a antibody cocktail (clone JCB117 + HM47/A9) as confirmation of integrity and purity.

  • CD79a Antibody Cocktail [orb2637633]

    FACS,  IF,  IHC-P,  WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg