CD79a Antibody

SKU: orb749344

Description

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B-cell Ag receptor. CD79a first appears at pre B-cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. It is found in the majority of acute leukemias of B-cell precursors, lines and lymphomas, and in some myelomas. It is not present in myeloid or Tcell lines. CD79a antibody is generally used to complement CD20 mAb, especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This CD79a antibody will stain many of the same lymphomas as CD20 mAb, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is CD20 mAb. CD79a antibody also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD79a antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: JCB117
• Immunogen: A soluble form of the extracellular IgSF domain from the human protein was used as the immunogen for the CD79a antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

Immunofluorescent staining of PFA-fixed human Raji cells with CD79 antibody (clone JCB117, green) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with CD79a antibody (clone JCB117); Red = isotype control, Blue = CD79a antibody.

SDS-PAGE analysis of purified, BSA-free CD79a antibody (clone JCB117) as confirmation of integrity and purity.

Western blot testing of two different lots of human Raji cell lysate with CD79a antibody (clone JCB117). Expected molecular weight: 25-47 kDa depending on glycosylation level.