Anti-CD20 [10F381 (rituximab)]

SKU: orb396140

Description

Mouse monoclonal antibody to CD20.

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Human, Primate

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 10F381 (rituximab)
• Immunogen: Human lymphoblastoid cell line SB.
• Target: CD20
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Leu16; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35 Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD20

Flow-cytometry using the anti-CD20 research biosimilar antibody Rituximab Cynomolgus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Rituximab (orb378213, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using the anti-CD20 research biosimilar antibody Rituximab Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Rituximab (orb378213, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using the anti-CD20 research biosimilar antibody Rituximab Rhesus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Rituximab (orb378213, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

CD20

UniProt

P11836

References

Reff, M. E. et al. Depletion of B cells in vivo by a chimeric mouse human monoclonal antibody to CD20 Blood, 83, 435-445 (1994)

PubMed: 7506951