RHCG Antibody

SKU: orb1249041

Description

RHCG Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Testis lysates (calculated MW of 53.2kDa according to NP_057405.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Biol Chem. 2000 Aug 18;275(33):25641-51;PMID: 10852913). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Kidney. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ERQNSVYQSD
• Target: RHCG
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RHCG, Rh family, C glycoprotein, C15orf6, PDRC2, RHGK, SLC42A3, Rh type C glycoprotein, Rhesus blood group, C glycoprotein

orb1249041 (1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249041 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249041 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RHCG

UniProt

Q9UBD6

RefSeq (Protein):NP_057405.1