RHCG Antibody

• Catalog Number: orb1249041
• Category: Antibodies
• Description: RHCG Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-ERQNSVYQSD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Testis lysates (calculated MW of 53.2kDa according to NP_057405.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Biol Chem. 2000 Aug 18;275(33):25641-51;PMID: 10852913). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Kidney. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RHCG
• UniProt ID: Q9UBD6
• NCBI: NP_057405.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RHCG, Rh family, C glycoprotein, C15orf6, PDRC2, R
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Testis lysates (calculated MW of 53.2kDa according to NP_057405.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Biol Chem. 2000 Aug 18;275(33):25641-51;PMID: 10852913). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249041 (1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249041 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249041 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.