RFP antibody

• Catalog Number: orb420218
• Category: Antibodies
• Description: RFP antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
• Concentration: 1 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IF: User Optimized, WB: 1:1000-1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
• Conjugation: Unconjugated
• UniProt ID: Q9U6Y8
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-RFP antibody, DsRed, rDsRed, Discosoma s
• Note: For research use only
• Application notes: Polyclonal anti-RFP has been tested by SDS-Page and western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27kDa in Western Blot in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.