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RFC1 Antibody

Catalog Number: orb1259081

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb1259081
CategoryAntibodies
DescriptionRFC1 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human RFC1 (NP_001191676.1).
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MWObserved: 130kDa
TargetRFC1
UniProt IDP35251
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Alternative namesReplication factor C subunit 1, Activator 1 140 kD
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NoteFor research use only
Application notesWB: 1:500 - 1:2000IF: 1:50 - 1:200
Expiration Date12 months from date of receipt.
RFC1 Antibody

Western blot analysis of extracts of MCF-7 cells, using RFC1 antibody (orb1259081) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

RFC1 Antibody

Immunofluorescence analysis of MCF-7 cells using RFC1 antibody (orb1259081). Blue: DAPI for nuclear staining.

RFC1 Antibody

Immunofluorescence analysis of A549 cells using RFC1 antibody (orb1259081).

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