RETNLB Antibody

SKU: orb1246501

Description

RETNLB Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Ileum and Duodenum lysates after 0.1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to NP_115968.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Colon shows textured staining of cytoplasm in enterocytes. Recommended concentration, 2-4µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DSVMDKKIKDVLNS
• Target: RETNLB
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RETNLB , resistin like beta , FIZZ1, FIZZ2, HXCP2, RELM-beta, RELMb, RELMbeta, XCP2, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2, colon and small intestine-specific cysteine-rich protein, cysteine-rich secreted A12-alpha-like protein 1, found in inflammatory zone 1

orb1246501 (2.5g/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RETNLB

UniProt

Q9BQ08

RefSeq (Protein):NP_115968.1