RETNLB Antibody

SKU: orb1272785

Description

RETNLB Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Indirect:To detect hRELMb by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRELMb. Sandwich:To detect hRELMb by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot:To detect hRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMb. Human RELMb specific antibody was purified by affinity chromatography employing immobilized RELMb matrix.
• Target: RETNLB
• Purification: RELMb antibody was purified by affinity chromatography employing immobilized RELMb matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

XCP2, FIZZ1, FIZZ2, HXCP2, RELMb, RELMbeta, RELM-beta, CCRG, RETNL2, UNQ408/PRO770, Resistin-like beta, Colon and small intestine-specific cysteine-rich protein,

To detect Human RELM-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human RELM-beta (orb1272786) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human RELM-beta.

To detect Human RELM-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human RELM-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

RETNLB

UniProt

Q9BQ08

RefSeq:Q9BQ08