RELA Antibody (N-term)

SKU: orb37069

Description

Rabbit polyclonal antibody to NFkB p65

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Metabolism Research, Microbiology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:2000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Other, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD42908
• Immunogen: Synthetic Peptide
• Target: RELA
• Molecular Weight: 60219
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NF kappa B p65delta3 antibody, anti NFKB 3 antibody, anti NFKB3 antibody, anti Nuclear Factor NF Kappa B p65 Subunit antibody, anti Nuclear factor NF-kappa-B p65 subunit antibody, anti Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody, anti Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody, anti Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody, anti p65 antibody, anti p65 NF kappaB antibody, anti p65 NFkB antibody, anti RELA antibody, anti NF-kB p65 antibody, anti NFKBp65 antibody, anti NF-kBp65 antibody, anti NF-kBp65 antibody, anti p65 antibody, anti p65 NF kappaB antibody, anti p65 NFkB antibody, anti RELA antibody, anti Transcription Factor p65 antibody, anti p65NFKB antibody

RELA Antibody (N-term) western blot analysis in Hela, MCF-7, Raji, Ramos cell line lysates (35 ug/lane). This demonstrates the RELA antibody detected the RELA protein (arrow).

Confocal immunofluorescent analysis of RELA Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

RELA Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with RELA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-RELA Antibody (N-term) at 1:2000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 2: THP-1 whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

RELA

UniProt

Q04206

RefSeq (Protein):NP_001138610.1, NP_068810.3, NP_001230913.1

M Vaivads, M Pilmane - Diagnostics Distribution of Immunomodulation, Protection and Regeneration Factors in Cleft-Affected Bone and Cartilage Diagnostics, (2024)

PubMed:

Applications

IHC

Reactivity

Human