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DsRed Antibody

SKU: orb1463559

Description

Recombinant RFP antibody

Images & Validation

Tested ApplicationsDOT, ELISA, WB
Dilution RangeELISA: 1:31,000, WB: 1:1000
ReactivityOther
Application Notes
Anti-RFP is a his-tagged monoclonal recombinant antibody designed to detect Red Fluorescent Protein and its conjugates. This antibody has been tested by dot blot and ELISA and is intended for use in immunological assays including ELISA, immunofluorescence and fluorescence activated cell sorting (FACS). The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of RFP-conjugated molecules by immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user. This antibody is not suitable for Western blot detection of denatured RFP.

Key Properties

Antibody TypePrimary Antibody
ClonalityRecombinant
Clone No.RFP40
TargetDsRed
Source/Expression SystemE. coli
PurityAnti-RFP (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against RFP-labeled probes and proteins.
ConjugationUnconjugated

Storage & Handling

StorageStore at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 20% (v/v) Glycerol; Buffer: 1X PBS, pH 7.4
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

recombinant anti-RFP antibody, Red Fluorescent Protein antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
DOT
Dot Blot
View Protocol

Available Sizes

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50 μg
Free Secondary Antibody (20 ul)0/0

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