Recombinant RFP antibody

• Catalog Number: orb1463559
• Category: Antibodies
• Description: Recombinant RFP antibody
• Clonality: Recombinant
• Clone Number: RFP40
• Tested applications: DOT, ELISA, WB
• Reactivity: Other
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:31,000, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-RFP (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against RFP-labeled probes and proteins.
• Conjugation: Unconjugated
• UniProt ID: Q9U6Y8
• Source: E. Coli
• Storage: Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: recombinant anti-RFP antibody, Red Fluorescent Pro
• Note: For research use only
• Application notes: Anti-RFP is a his-tagged monoclonal recombinant antibody designed to detect Red Fluorescent Protein and its conjugates. This antibody has been tested by dot blot and ELISA and is intended for use in immunological assays including ELISA, immunofluorescence and fluorescence activated cell sorting (FACS). The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of RFP-conjugated molecules by immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user. This antibody is not suitable for Western blot detection of denatured RFP.
• Expiration Date: 12 months from date of receipt.