Insect Baculovirus Expression System

The Insect Baculovirus Expression Vector System (BEVS) is categorized as a eukaryotic expression system, distinguished by its high safety standards. With its expansive genome, it accommodates the insertion of large exogenous genes, offering a significant advantage in expressing proteins with substantial molecular weights.

Furthermore, it possesses the capability to accomplish full post-translational modifications and effectively express exogenous genes. The system comprises a transfer vector, baculovirus vector, and host cell components. Employing one or more potent baculovirus super-strong promoters, the system generates recombinant viruses by inserting the exogenous target gene into the promoter. This process achieves highly efficient expression of the exogenous gene as the recombinant viruses replicate within insect cells.

BEVS is commonly employed in the development of virus vaccines, such as those for influenza and HPV. Additionally, it is utilised in the production of cell signalling proteins, cytokines, and kinase development, among other applications.

Service Process & Deliverables

1. Plasmid Construction

15 – 20 Days

Workflow Process

• Target sequence codon optimization and custom gene synthesis
• PCR product ligation into specialized baculovirus expression vectors
• Transformation into Top 10 E. coli cells and positive colony screening
• Sequence verification to deliver sequence-confirmed recombinant plasmids

Key Feature

Vector Optimization: To enhance expression success and yield, we offer protein expression with C-terminal fusion tags alongside conventional N-terminal tags. This approach maintains protein bioactivity while ensuring high purity.

2. Bacmid & High-Titer Virus

15 – 20 Days

Workflow Process

• Transform DH10Bac cells to obtain recombinant Bacmid; verify via PCR analysis and isolate recombinant Bacmid DNA
• Transfect insect cells with recombinant Bacmid DNA to generate initial baculovirus stock
• Assess expression levels via SDS-PAGE and repeat infection cycles as necessary to amplify viral titer

Key Feature

Suspension Transfection: Our distinctive suspension transfection method significantly enhances protein expression levels and efficiently shortens the experimental timeframe.

3. Scale-Up & Purification

5 – 10 Days

Workflow Process

• Infect large-scale suspension insect cell cultures with optimized baculovirus stock
• Purify target protein via affinity chromatography, ion exchange, hydrophobic interaction, and molecular sieves

Key Feature

Following optimization, substantial quantities of high-purity protein are acquired by infecting host cells with low-passage virus to prevent genetic instability.

4. Optional Services

5 Days

Available Options

• Endotoxin removal (<0.1 EU/µg or <0.01 EU/µg thresholds)
• Aseptic processing, 0.22 µm filter sterilization, and custom aliquoting
• Lyophilization for extended shelf-life stability
• Enzymatic tag removal and cleavage (optional add-on)

Key Feature

Customers have the flexibility to select from a range of additional downstream services tailored to their specific analytical or therapeutic requirements.

5. QC & Release

5 Days

Workflow Process

• Purity determination via SDS-PAGE and HPLC (>85% to >98%)
• Accurate concentration quantification (BCA or A280 absorption)
• Optional SEC-MALS or bioactivity verification assays

Key Feature

Every delivered project includes a comprehensive Certificate of Analysis (COA) documenting experimental conditions and analytical QC chromatograms.

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