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The mammalian expression system is a vital tool in biotechnology and medicine, allowing the production of complex proteins with native-like properties. It's widely used for therapeutic protein production and research due to its ability to mimic human biology closely. Continuous improvements in technology enhance its efficiency and scalability, while regulatory standards ensure product safety and efficacy. Ongoing research aims to optimise expression systems and develop new therapeutic proteins, supported by advancements in genome editing.
- Proper Protein Folding: Mammalian cells have the machinery to fold proteins correctly, ensuring functional activity.
- Post-Translational Modifications (PTMs): Mammalian cells can perform PTMs like glycosylation and phosphorylation, crucial for many proteins' function and stability.
- Relevance to Human Biology: Mammalian cells closely mimic human cell biology, making them ideal for producing proteins intended for therapeutic use or studying human proteins.
- Complex Protein Expression: Mammalian cells can handle the expression of complex proteins that require specific folding and PTMs, which may not be possible in simpler expression systems like bacteria or yeast.
- Low Immunogenicity: Proteins produced in mammalian cells are less likely to induce an immune response when used in therapeutic applications compared to those produced in prokaryotic systems.
Service Process
1. Plasmid construction
Process
- Codon optimization; gene synthesis
- The PCR product is ligated to the expression vectors
- Top 10 E.coli cells are transformed
- Obtain the correct recombinant plasmid
Features
Vector optimization - To enhance expression success and yield, we offer protein expression with C-terminal fusion tags alongside conventional N-terminal tags. This approach maintains protein bioactivity while ensuring high purity
Lead Time: 15-20 business days
2. Small scale expression
Process
- Prepare the transfection grade recombinant plasmid in larger quantities
- Transient transfect HEK293, CHO and other cells
- Detect expression products
Features
Optimization of transfection conditions - Test various transfection conditions and choose the most suitable experimental parameters based on the results
Lead Time: 10-15 business days
3. Scale up expression and purification
Process
- Scale up the culture cells and transfect
- Different chromatographic conditions are explored to determine the optimal purification method
Features
Multi-condition expression scheme - Based on the protein's localization and the optimal experimental conditions identified during initial small-scale expression tests, cell lines and transfection methods are chosen to enhance protein expression quantity, significantly improving overall protein expression levels
Lead Time: 10-15 business days
4. Optional additional services
Process
- Endotoxin removal, lyophilisation or filter-sterilisation
- Tag removal (Additional charge)
Features
Customers have the flexibility to select from a range of additional services tailored to their specific requirements
Lead Time: 5 business days
5. Quality control
Process
The purity, concentration and further tests are performed
Features
Detailed COA report is provided