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Catalog Number | orb1410350 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to p53 Tumor Suppressor Protein |
Target | TP53 |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG2a |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Buffer/Preservatives | 200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Immunogen | Recombinant full-length human TP53 protein |
UniProt ID | P04637 |
MW | 53kDa. |
Tested applications | IHC |
Dilution range | Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
Application notes | Positive Control: MDA-MB-231 cells. Human breast or colon carcinoma |
Antibody Type | Recombinant Antibody |
Clone Number | rTP53/6927 |
Expression System | CHO cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53 Read more... |
Note | For research use only |
Entrez | 7157 |
FC, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |