Cart summary

You have no items in your shopping cart.

P53 (FL-393) Rabbit Polyclonal Antibody

Catalog Number: orb2664

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb2664
CategoryAntibodies
DescriptionP53 (FL-393) Rabbit Polyclonal Antibody
TargetTP53
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Equine, Porcine, Sheep
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenFull length p53 protein of human origin (1-393/393aa)
UniProt IDP04637
MW53 kDa
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesLFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor S
Read more...
NoteFor research use only
P53 (FL-393) Rabbit Polyclonal Antibody

Sample: MCF-7 Cell (Human) Lysate at 40 ug, Primary: Anti-p53 (FL-393) (orb2664) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 60 kD.

P53 (FL-393) Rabbit Polyclonal Antibody

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (p53 (FL-393)) polyclonal Antibody, Unconjugated (orb2664) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

P53 (FL-393) Rabbit Polyclonal Antibody

Blank control: A549. Primary Antibody (green line): Rabbit Anti-p53 (FL-393) antibody (orb2664), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

P53 (FL-393) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p53 (FL-393)) Polyclonal Antibody, Unconjugated (orb2664) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

P53 (FL-393) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p53 (FL-393)) Polyclonal Antibody, Unconjugated (orb2664) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

P53 (FL-393) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p53 (FL-393)) Polyclonal Antibody, Unconjugated (orb2664) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

P53 (FL-393) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Transformation related protein 53, P53) Polyclonal Antibody, Unconjugated (orb2664) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

P53 (FL-393) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Transformation related protein 53, P53) Polyclonal Antibody, Unconjugated (orb2664) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

P53 (FL-393) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p53 (FL-393)) Polyclonal Antibody, Unconjugated (orb2664) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

P53 (FL-393) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Transformation related protein 53, P53) Polyclonal Antibody, Unconjugated (orb2664) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

P53 (FL-393) Rabbit Polyclonal Antibody

Sample: 293T Cell (Human) Lysate at 40 ug, Primary: Anti-p53 (FL-393) (orb2664) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 60 kD.

  • P53 (FL-393) Rabbit Polyclonal Antibody (HRP) [orb481883]

    IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Equine, Porcine, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μl
  • P53 (FL-393) Rabbit Polyclonal Antibody (PE-Cy5.5) [orb875351]

    FC,  ICC,  IF

    Bovine, Canine, Equine, Porcine, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE/Cy5.5

    100 μl
  • P53 (FL-393) Rabbit Polyclonal Antibody (PE-Cy7) [orb875022]

    FC,  ICC,  IF

    Bovine, Canine, Equine, Porcine, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE/Cy7

    100 μl
  • P53 (FL-393) Rabbit Polyclonal Antibody (Cy5) [orb873192]

    FC,  ICC,  IF

    Bovine, Canine, Equine, Porcine, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Cy5

    100 μl
  • P53 (FL-393) Rabbit Polyclonal Antibody (AP) [orb873522]

    IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Equine, Porcine, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    AP

    100 μl