Human TSLP

SKU: orb2993808

Description

Recombinant Human Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159(R127A, R130A) is expressed with a 10His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-Human TSLP mAb(Cat#orb2992721) at 2μg/ml (100 μl/well)can bind Human TSLP-His(Cat#orb2993808). The ED50 of Human TSLP-His(Cat#orb2993808)is 4.48 ng/ml.. BLI: Loaded Anti-Human TSLP mAb-Fc(Cat#orb2992721) on Protein A Biosensor, can bind Human TSLP-His(Cat#orb2993808) with an affinity constant of -3 nM as determined in BLI assay.
• Tag: C-10His
• Molecular Weight: Predicted: 16.1 KDa. Observed: 20-27 KDa, reducing conditions
• Expression Region: Tyr29-Gln159(R127A, R130A)
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 4% Sucrose, 4% Mannitol, 0.02% Tween 80 (w/v), pH 6.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thymic stromal lymphopoietin; TSLP

Quick Database Links

UniProt

Q969D9