Biotinylated Human TSLP

SKU: orb2992987

Description

Biotinylated Recombinant Human Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159 is expressed with a 10His, Avi tag at the C-terminus.

Key Properties

• Expression System: Human cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-Human TSLP mAb-Fc (Cat#orb2992721) at 1μg/ml (100 μl/well) can bind Biotinylated Human TSLP-His-Avi (Cat#orb2992987).The ED50 of Biotinylated Human TSLP-His-Avi(Cat#orb2992987) is 0.851 ng/ml. (Regularly tested). BLI: Loaded Biotinylated Human TSLP-His-Avi (Cat#orb2992987) on SA Biosensor, can bind Anti-Human TSLP mAb (Cat#orb2992721) with an affinity constant of 46.9 pM as determined in BLI assay. (Regularly tested)
• Tag: C-10His-Avi
• Molecular Weight: Predicted: 18.1 kDa. Observed: (10-13)&(16-29) kDa, reducing conditions
• Expression Region: Tyr29-Gln159
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 95% as determined by SEC-HPLC.(QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; TSLP

Quick Database Links

UniProt

Q969D9