Recombinant Human TSLP Protein

SKU: orb2994446

Description

Recombinant Human Thymic Stromal Lymphopoietin is produced by our E.coli expression system and the target gene encoding Tyr29-Gln159 is expressed.

Key Properties

• Expression System: E.coli
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human TSLP (Cat#orb2994446) at 10μg/ml (100 μl/well) can bind Human TSLP R-Fc (Cat#orb2993863).The ED50 of Human TSLP R-Fc (Cat#orb2993863) is 0.16 ug/ml.. BLI: Loaded Human TSLP R-Fc(Cat#orb2993863) on Protein A Biosensor, can bind Human TSLP(Cat#orb2994446) with an affinity constant of 9.04 nM as determined in BLI assay. Loaded Anti-Human TSLP mAb-Fc(Cat#orb2992721) on Protein A Biosensor, can bind Human TSLP(Cat#orb2994446) with an affinity constant of 0.01 nM as determined in BLI assay.
• Tag: Tag free
• Expression Region: Tyr29-Gln159
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 15.1 KDa. Observed: 14 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thymic stromal lymphopoietin; TSLP

Quick Database Links

UniProt

Q969D9