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Recombinant Human TSLP Protein
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human TSLP (Cat#orb2994446) at 10μg/ml (100 μl/well) can bind Human TSLP R-Fc (Cat#orb2993863).The ED50 of Human TSLP R-Fc (Cat#orb2993863) is 0.16 ug/ml.. BLI: Loaded Human TSLP R-Fc(Cat#orb2993863) on Protein A Biosensor, can bind Human TSLP(Cat#orb2994446) with an affinity constant of 9.04 nM as determined in BLI assay. Loaded Anti-Human TSLP mAb-Fc(Cat#orb2992721) on Protein A Biosensor, can bind Human TSLP(Cat#orb2994446) with an affinity constant of 0.01 nM as determined in BLI assay. |
| Tag | Tag free |
| Molecular Weight | Predicted: 15.1 KDa. Observed: 14 KDa, reducing conditions |
| Expression Region | Tyr29-Gln159 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Recombinant Human TSLP Protein (orb2994446)
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