Recombinant Human TSLP Protein

SKU: orb2992988

Description

Recombinant Human Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human TSLP R-His(Cat#orb2993884) at 2μg/ml (100 μl/well) can bind Human TSLP-Fc(Cat#orb2992988).The ED50 of Human TSLP-Fc(Cat#orb2992988) is 1.32 ng/ml. (Regularly tested). BLI: Loaded Human TSLP-Fc(Cat#orb2992988) on Protein A Biosensor, can bind Human TSLPR&IL-7RA Heterodimer-His(Cat#orb2993098) with an affinity constant of 0.50 nM as determined in BLI assay. (Regularly tested) Loaded Human TSLP-Fc(Cat#orb2992988) on Protein A Biosensor, can bind Human TSLP R-His(Cat#orb2993884) with an affinity constant of 0.24 uM as determined in BLI assay. (Regularly tested)
• Tag: C-Fc
• Expression Region: Tyr29-Gln159
• Conjugation: Unconjugated
• MW: Predicted: 41.9 kDa. Observed: 45-58 kDa
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified)

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; TSLP

Quick Database Links

UniProt

Q969D9